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ADA ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Adenosine deaminase

Gene Aliases

Adenosine aminohydrolase; adenosine deaminase.

Gene ID

11486

Accession Number

NP_001258981.1

Reactivity

Mouse, Mus musculus

Target

Adenosine deaminase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ada ELISA Kit (Mouse) (OKEH05529) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ada has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ada is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ada captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.

Detection Range

78-5000pg/mL

Sensitivity

40.5 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Adenosine deaminase

Protein Length

352

NCBI Gene Symbol

Ada

Protein Name

P03958

Gene Name URL

Ada

Nucleotide Accession Number

NM_001272052.1

Frequently Asked Questions

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