CA14 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Carbonic anhydrase 14
Gene Aliases
AW536446; CA XIV; Ca14; carbonate dehydratase XIV; carbonic anhydrase 14; carbonic anhydrase XIV; CA-XIV.
Gene ID
23831
Accession Number
NP_035927.1
Reactivity
Mouse, Mus musculus
Target
Carbonic anhydrase 14
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ca14 ELISA Kit (Mouse) (OKEH05506) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ca14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ca14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ca14 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 97.6%
Sensitivity
0.178 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Carbonic anhydrase 14
Protein Length
337
NCBI Gene Symbol
Car14
Protein Name
Q9WVT6
Gene Name URL
Car14
Nucleotide Accession Number
NM_011797.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
3321 1/pk
CELLSTACK CHAMBER,CORNING CELLBIND SURFACE,40-STACK,PS,S,IND,1/2
Sign In for Pricing
View Details 
TSD-381114TT-70 500pcs/bag,2bags/ctn
381 x 114mm;34oz/1000ml; sterile;0.07 mm (single-sided)
Sign In for Pricing
View Details MBS6357828-01 0.2 mL
TRADD, ID (TRADD, Tumor necrosis factor receptor type 1-associated DEATH domain protein, TNFRSF1A-associated via death domain) (Azide free) (HRP)
Sign In for Pricing
View Details MBS6357828-02 5x 0.2 mL
TRADD, ID (TRADD, Tumor necrosis factor receptor type 1-associated DEATH domain protein, TNFRSF1A-associated via death domain) (Azide free) (HRP)
Sign In for Pricing
View Details 
bs-12224R-BF594 100 µL
ZNF852 Polyclonal Antibody, AbBy Fluor™ 594 Conjugated
Sign In for Pricing
View Details