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PIK3CD ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta

Gene Aliases

2410099E07Rik;2610208K16Rik; AW545373; catalytic phosphatidylinositol 3-kinase delta; p100 delta; p110de; p110delta; phosphatidylinositol 3-kinase catalytic delta polypeptide; phosphatidylinositol 3-kinase catalytic subunit delta; phosphatidylinositol 3-kinase p110 delta; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphoinositide 3 kinase P110delta; PI3Kdelta; PI3K-delta; PI3-kinase subunit delta; ptdIns-3-kinase subunit delta; ptdIns-3-kinase subunit p110-delta.

Gene ID

18707

Accession Number

NP_001157524.1

Reactivity

Mouse, Mus musculus

Target

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pik3cd ELISA Kit (Mouse) (OKEH05498) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pik3cd has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pik3cd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pik3cd captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 90%

Sensitivity

0.184 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform

Protein Length

1043

NCBI Gene Symbol

Pik3cd

Protein Name

O35904

Gene Name URL

Pik3cd

Nucleotide Accession Number

NM_001164052.1

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