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CPM ELISA Kit (Mouse)

Selected Variant

OKEH05484-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Carboxypeptidase M

Gene Aliases

1110060I01Rik;5730456K23Rik; AA589379; carboxypeptidase M; E030045M14Rik.

Gene ID

70574

Accession Number

NP_081744.1

Reactivity

Mouse, Mus musculus

Target

Carboxypeptidase M

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Cpm ELISA Kit (Mouse) (OKEH05484) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cpm has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cpm is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cpm captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 102.1%

Sensitivity

0.802 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Carboxypeptidase M

Protein Length

443

NCBI Gene Symbol

Cpm

Protein Name

Q80V42

Gene Name URL

Cpm

Nucleotide Accession Number

NM_027468.1

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