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LAMP2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Lysosomal-associated membrane protein 2

Gene Aliases

CD107 antigen-like family member B; CD107b; Lam; Lamp; Lamp II; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; LGP-B; lysosomal membrane glycoprotein 2; lysosomal membrane glycoprotein type B; lysosome-associated membrane glycoprotein 2; Mac; Mac3.

Gene ID

16784

Accession Number

NP_001017959.1

Reactivity

Mouse, Mus musculus

Target

Lysosome-associated membrane glycoprotein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Lamp2 ELISA Kit (Mouse) (OKEH05478) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lamp2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lamp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lamp2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 91.7%

Sensitivity

0.182 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Lysosome-associated membrane glycoprotein 2

Protein Length

415

NCBI Gene Symbol

Lamp2

Protein Name

P17047

Gene Name URL

Lamp2

Nucleotide Accession Number

NM_001017959.2

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