BMP15 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Bone morphogenetic protein 15
Gene Aliases
AU015375; AU018861; AU021453; Bmp-1; Bmp-15; bone morphogenetic protein 15; C86824; C87336; GDF-9B; growth/differentiation factor 9B.
Gene ID
12155
Accession Number
NP_033887.1
Reactivity
Mouse, Mus musculus
Target
Bone morphogenetic protein 15
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Bmp15 ELISA Kit (Mouse) (OKEH05476) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmp15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bmp15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bmp15 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99.7%
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Bone morphogenetic protein 15
Protein Length
392
NCBI Gene Symbol
Bmp15
Protein Name
Q9Z0L4
Gene Name URL
Bmp15
Nucleotide Accession Number
NM_009757.5
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog