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DDX42 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

DEAD box helicase 42

Gene Aliases

1810047H21Rik; ATP-dependent RNA helicase DDX42; AW319508; AW556242; B430002H05Rik; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box protein 42; RHELP; RNA helicase-related protein; RNAHP; SF3b12; SF3b125.

Gene ID

72047

Accession Number

NP_082350.3

Reactivity

Mouse, Mus musculus

Target

ATP-dependent RNA helicase DDX42

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ddx42 ELISA Kit (Mouse) (OKEH05469) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ddx42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ddx42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ddx42 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 81.4%

Sensitivity

15.78 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse ATP-dependent RNA helicase DDX42

Protein Length

929

NCBI Gene Symbol

Ddx42

Protein Name

Q810A7

Gene Name URL

Ddx42

Nucleotide Accession Number

NM_028074.4

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