DDX42 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
DEAD box helicase 42
Gene Aliases
1810047H21Rik; ATP-dependent RNA helicase DDX42; AW319508; AW556242; B430002H05Rik; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box protein 42; RHELP; RNA helicase-related protein; RNAHP; SF3b12; SF3b125.
Gene ID
72047
Accession Number
NP_082350.3
Reactivity
Mouse, Mus musculus
Target
ATP-dependent RNA helicase DDX42
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ddx42 ELISA Kit (Mouse) (OKEH05469) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ddx42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ddx42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ddx42 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 81.4%
Sensitivity
15.78 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse ATP-dependent RNA helicase DDX42
Protein Length
929
NCBI Gene Symbol
Ddx42
Protein Name
Q810A7
Gene Name URL
Ddx42
Nucleotide Accession Number
NM_028074.4
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