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UTS2B ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Urotensin 2B

Gene Aliases

U, U2B, Urp, Uts2, Gm538, Uts2d

Gene ID

224065

Accession Number

NP_937809.1

Reactivity

Mouse

Target

Potent vasoconstrictor.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Uts2b ELISA Kit (Mouse) (OKEH05465) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Uts2b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Uts2b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Uts2b captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78 - 5000pg/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 84.8%

Sensitivity

39.29 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Urotensin-2B

Protein Length

113

NCBI Gene Symbol

UTS2B

Protein Name

Urotensin-2B

Gene Name URL

UTS2B

Nucleotide Accession Number

NM_198166.3

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