ADRA2A ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Adrenoceptor alpha 2A
Gene Aliases
ADRA2; ADRA2R; ADRAR; adrenergic, alpha-2A-, receptor; alpha-2 adrenergic receptor subtype C10; alpha-2A adrenergic receptor; alpha-2A adrenoceptor; alpha-2A adrenoreceptor; ALPHA2AAR; alpha-2AAR subtype C10; alpha-2-adrenergic receptor, platelet type; ZNF32.
Gene ID
150
Accession Number
NP_000672.3
Reactivity
Homo sapiens, Human
Target
Alpha-2A adrenergic receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ADRA2A ELISA Kit (Human) (OKEH05464) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADRA2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADRA2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADRA2A captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 87%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Alpha-2A adrenergic receptor
Protein Length
450
NCBI Gene Symbol
ADRA2A
Protein Name
P08913
Gene Name URL
ADRA2A
Nucleotide Accession Number
NM_000681.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog