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ANG2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Angiogenin, ribonuclease A family, member 2

Gene Aliases

A; angiogenin related protein; angiogenin-2; Angiogenin-related protein; Angrp; Raa3; ribonuclease A a3; Rna; Rnase5b.

Gene ID

11731

Accession Number

NP_031475.2

Reactivity

Mouse, Mus musculus

Target

Angiogenin-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ang2 ELISA Kit (Mouse) (OKEH05460) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ang2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ang2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ang2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 97.1%

Sensitivity

15.6 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Angiogenin-2

Protein Length

145

NCBI Gene Symbol

Ang2

Protein Name

Q64438

Gene Name URL

Ang2

Nucleotide Accession Number

NM_007449.2

Frequently Asked Questions

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