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BCL11B ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

B cell leukemia/lymphoma 11B

Gene Aliases

9130430L19Rik; AI604821; B630002E05Rik; B-cell CLL/lymphoma 11B; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; BCL-11B; COUP-TF-interacting protein 2; CTI; Ctip2; mRit1; radiation-induced tumor suppressor gene 1 protein; Ri; Rit1.

Gene ID

58208

Accession Number

NP_001073352.1

Reactivity

Mouse, Mus musculus

Target

B-cell lymphoma/leukemia 11B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Bcl11b ELISA Kit (Mouse) (OKEH05455) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bcl11b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bcl11b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bcl11b captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 96.8%

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse B-cell lymphoma/leukemia 11B

Protein Length

884

NCBI Gene Symbol

Bcl11b

Protein Name

Q99PV8

Gene Name URL

Bcl11b

Nucleotide Accession Number

NM_001079883.1

Frequently Asked Questions

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