NT5C3B ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
5'-nucleotidase, cytosolic IIIB
Gene Aliases
Nt5c, Nt5c3l, AI841843, 2610037D24Rik, C330027I04Rik
Gene ID
68106
Accession Number
NP_001096120.1
Reactivity
Mouse
Target
Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Nt5c3b ELISA Kit (Mouse) (OKEH05452) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nt5c3b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nt5c3b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nt5c3b captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312 - 20ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 93.1%
Sensitivity
0.18 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Cytosolic 5'-nucleotidase III-like protein
Protein Length
300
NCBI Gene Symbol
NT5C3B
Protein Name
7-methylguanosine phosphate-specific 5'-nucleotidase
Gene Name URL
NT5C3B
Nucleotide Accession Number
NM_001102650.1
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