Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

EPHB4 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

EPH receptor B4

Gene Aliases

CMAVM2; ephrin receptor EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; HFASD; HTK; LMPHM7; MYK1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; TYRO11; tyrosine-protein kinase receptor HTK; tyrosine-protein kinase TYRO11.

Gene ID

2050

Accession Number

NP_004435.3

Reactivity

Homo sapiens, Human

Target

Ephrin type-B receptor 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology EPHB4 ELISA Kit (Human) (OKEH05438) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPHB4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPHB4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EPHB4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 95%

Sensitivity

8.04 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Ephrin type-B receptor 4

Protein Length

987

NCBI Gene Symbol

EPHB4

Protein Name

P54760

Gene Name URL

EPHB4

Nucleotide Accession Number

NM_004444.4

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

FOXP3 (3G3), CF647 conjugate, 0.1mg/mL
BNC470645-500 1x 500 µL

FOXP3 (3G3), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
HSP27 (G3.1), CF647 conjugate, 0.1mg/mL
BNC470861-500 1x 500 µL

HSP27 (G3.1), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD61 / Integrin 3 / Platelet Glycoprotein IIIa (Platelet Marker) (ITGB3/2145), CF568 conjugate, 0.1mg/mL
BNC682145-500 1x 500 µL

CD61 / Integrin 3 / Platelet Glycoprotein IIIa (Platelet Marker) (ITGB3/2145), CF568 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
MiTF (Microphthalmia Transcription Factor) (D5 + MITF/915), CF647 conjugate, 0.1mg/mL
BNC470950-500 1x 500 µL

MiTF (Microphthalmia Transcription Factor) (D5 + MITF/915), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD2 (LFA2/600), CF740 conjugate, 0.1mg/mL
BNC740600-500 1x 500 µL

CD2 (LFA2/600), CF740 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
MHC II (HLA-DRA) (19-26.1), CF640R conjugate, 0.1mg/mL
BNC401082-500 1x 500 µL

MHC II (HLA-DRA) (19-26.1), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details