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LRRK2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Leucine-rich repeat kinase 2

Gene Aliases

4921513O20Rik;9330188B09Rik; AW561911; cI-4; cI-46; D630001M17Rik; Gm927; leucine-rich repeat serine/threonine-protein kinase 2.

Gene ID

66725

Accession Number

NP_080006.3

Reactivity

Mouse, Mus musculus

Target

Leucine-rich repeat serine/threonine-protein kinase 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Lrrk2 ELISA Kit (Mouse) (OKEH05425) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lrrk2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lrrk2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lrrk2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 82.5%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Leucine-rich repeat serine/threonine-protein kinase 2

Protein Length

2527

NCBI Gene Symbol

Lrrk2

Protein Name

Q5S006

Gene Name URL

Lrrk2

Nucleotide Accession Number

NM_025730.3

Frequently Asked Questions

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