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NOTUM ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Notum palmitoleoyl-protein carboxylesterase

Gene Aliases

5730593N15Rik; notum pectinacetylesterase homolog; palmitoleoyl-protein carboxylesterase NOTUM; protein notum homolog.

Gene ID

77583

Accession Number

NP_780472.3

Reactivity

Mouse, Mus musculus

Target

Palmitoleoyl-protein carboxylesterase NOTUM

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Notum ELISA Kit (Mouse) (OKEH05412) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Notum has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Notum is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Notum captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 97.9%

Sensitivity

40.2 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Palmitoleoyl-protein carboxylesterase NOTUM

Protein Length

503

NCBI Gene Symbol

Notum

Protein Name

Q8R116

Gene Name URL

Notum

Nucleotide Accession Number

NM_175263.4

Frequently Asked Questions

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