NOTUM ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Notum palmitoleoyl-protein carboxylesterase
Gene Aliases
5730593N15Rik; notum pectinacetylesterase homolog; palmitoleoyl-protein carboxylesterase NOTUM; protein notum homolog.
Gene ID
77583
Accession Number
NP_780472.3
Reactivity
Mouse, Mus musculus
Target
Palmitoleoyl-protein carboxylesterase NOTUM
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Notum ELISA Kit (Mouse) (OKEH05412) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Notum has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Notum is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Notum captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 97.9%
Sensitivity
40.2 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Palmitoleoyl-protein carboxylesterase NOTUM
Protein Length
503
NCBI Gene Symbol
Notum
Protein Name
Q8R116
Gene Name URL
Notum
Nucleotide Accession Number
NM_175263.4
Frequently Asked Questions
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