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ERMP1 ELISA Kit (Mouse)

Selected Variant

OKEH05404-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Endoplasmic reticulum metallopeptidase 1

Gene Aliases

Aminopeptidase Fxna; b2b2633C; b2b2633Clo; D19Ertd410; D19Ertd410e; D19Wsu12; D19Wsu12e; endoplasmic reticulum metallopeptidase 1; felix-ina; Fxna; mKIAA1815.

Gene ID

226090

Accession Number

NP_001074682.1

Reactivity

Mouse, Mus musculus

Target

Endoplasmic reticulum metallopeptidase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ermp1 ELISA Kit (Mouse) (OKEH05404) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ermp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ermp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ermp1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 88.7%

Sensitivity

0.192 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Endoplasmic reticulum metallopeptidase 1

Protein Length

898

NCBI Gene Symbol

Ermp1

Protein Name

Q3UVK0

Gene Name URL

Ermp1

Nucleotide Accession Number

NM_001081213.1

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