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MAPKAPK3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

MAPK activated protein kinase 3

Gene Aliases

3PK; chromosome 3p kinase; MAP kinase-activated protein kinase 3; MAPKAP kinase 3; MAPKAP3; MAPKAPK-3; MAPKAP-K3; MDPT3; mitogen-activated protein kinase-activated protein kinase 3; MK3; MK-3.

Gene ID

7867

Accession Number

NP_001230854.1

Reactivity

Homo sapiens, Human

Target

MAP kinase-activated protein kinase 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MAPKAPK3 ELISA Kit (Human) (OKEH05387) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAPKAPK3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAPKAPK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAPKAPK3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 90%

Sensitivity

41.7 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human MAP kinase-activated protein kinase 3

Protein Length

382

NCBI Gene Symbol

MAPKAPK3

Protein Name

Q16644

Gene Name URL

MAPKAPK3

Nucleotide Accession Number

NM_001243925.1

Frequently Asked Questions

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