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PPIL1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Peptidylprolyl isomerase like 1

Gene Aliases

CGI-124; cyclophilin like 1; cyclophilin-related gene 1; CYPL1; hCyPX; PCH14; peptidyl-prolyl cis-trans isomerase; peptidyl-prolyl cis-trans isomerase-like 1; peptidylprolyl isomerase (cyclophilin) -like 1; PPIase; rotamase PPIL1.

Gene ID

51645

Accession Number

NP_057143.1

Reactivity

Homo sapiens, Human

Target

Peptidyl-prolyl cis-trans isomerase-like 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PPIL1 ELISA Kit (Human) (OKEH05381) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPIL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPIL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPIL1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 106%

Sensitivity

0.192 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Peptidyl-prolyl cis-trans isomerase-like 1

Protein Length

166

NCBI Gene Symbol

PPIL1

Protein Name

Q9Y3C6

Gene Name URL

PPIL1

Nucleotide Accession Number

NM_016059.4

Frequently Asked Questions

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