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PGAM2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Phosphoglycerate mutase 2

Gene Aliases

BPG-dependent PGAM 2; GSD10; muscle-specific phosphoglycerate mutase; PGAMM; PGAM-M; phosphoglycerate mutase 2; phosphoglycerate mutase 2 (muscle) ; phosphoglycerate mutase isozyme M.

Gene ID

5224

Accession Number

NP_000281.2

Reactivity

Homo sapiens, Human

Target

Phosphoglycerate mutase 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PGAM2 ELISA Kit (Human) (OKEH05377) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PGAM2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PGAM2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PGAM2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 101%

Sensitivity

42.2 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Phosphoglycerate mutase 2

Protein Length

253

NCBI Gene Symbol

PGAM2

Protein Name

P15259

Gene Name URL

PGAM2

Nucleotide Accession Number

NM_000290.3

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