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MAP2K6 ELISA Kit (Human)

Selected Variant

OKEH05362-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Mitogen-activated protein kinase kinase 6

Gene Aliases

Dual specificity mitogen-activated protein kinase kinase 6; MAPK/ERK kinase 6; MAPKK 6; MAPKK6; MEK 6; MEK6; MKK6; PRKMK6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) ; SAPK kinase 3; SAPKK3; SAPKK-3; stress-activated protein kinase kinase 3.

Gene ID

5608

Accession Number

NP_002749.2

Reactivity

Homo sapiens, Human

Target

Dual specificity mitogen-activated protein kinase kinase 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MAP2K6 ELISA Kit (Human) (OKEH05362) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAP2K6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAP2K6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAP2K6 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 103%

Sensitivity

0.092 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Dual specificity mitogen-activated protein kinase kinase 6

Protein Length

334

NCBI Gene Symbol

MAP2K6

Protein Name

P52564

Gene Name URL

MAP2K6

Nucleotide Accession Number

NM_002758.3

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