COLGALT1 ELISA Kit (Mouse)
Selected Variant
OKEH05328-96W
Size: 96 Well
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Collagen beta (1-O) galactosyltransferase 1
Gene Aliases
2810024B22Rik; Collagen beta (1-O) galactosyltransferase 1; Glt25d; Glt25d1; glycosyltransferase 25 domain containing 1; glycosyltransferase 25 family member 1; hydroxylysine galactosyltransferase 1; procollagen galactosyltransferase 1.
Gene ID
234407
Accession Number
NP_666323.2
Reactivity
Mouse, Mus musculus
Target
Procollagen galactosyltransferase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Colgalt1 ELISA Kit (Mouse) (OKEH05328) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Colgalt1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Colgalt1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Colgalt1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 109%
Sensitivity
0.083 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Procollagen galactosyltransferase 1
Protein Length
617
NCBI Gene Symbol
Colgalt1
Protein Name
Q8K297
Gene Name URL
Colgalt1
Nucleotide Accession Number
NM_146211.3
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