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COLGALT1 ELISA Kit (Mouse)

Selected Variant

OKEH05328-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Collagen beta (1-O) galactosyltransferase 1

Gene Aliases

2810024B22Rik; Collagen beta (1-O) galactosyltransferase 1; Glt25d; Glt25d1; glycosyltransferase 25 domain containing 1; glycosyltransferase 25 family member 1; hydroxylysine galactosyltransferase 1; procollagen galactosyltransferase 1.

Gene ID

234407

Accession Number

NP_666323.2

Reactivity

Mouse, Mus musculus

Target

Procollagen galactosyltransferase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Colgalt1 ELISA Kit (Mouse) (OKEH05328) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Colgalt1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Colgalt1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Colgalt1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 109%

Sensitivity

0.083 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Procollagen galactosyltransferase 1

Protein Length

617

NCBI Gene Symbol

Colgalt1

Protein Name

Q8K297

Gene Name URL

Colgalt1

Nucleotide Accession Number

NM_146211.3

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