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NFKBIB ELISA Kit (Mouse)

Selected Variant

OKEH05317-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, beta

Gene Aliases

I (Kappa) B (beta) ; Ik; IKapp; I-kappa-B-beta; IKappaBbeta; IkB; ikB-B; IkBb; IKB-beta; NF-kappa-B inhibitor beta; NF-kappa-BIB; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta.

Gene ID

18036

Accession Number

NP_001293151.1

Reactivity

Mouse, Mus musculus

Target

NF-kappa-B inhibitor beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Nfkbib ELISA Kit (Mouse) (OKEH05317) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nfkbib has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nfkbib is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nfkbib captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 99.6%

Sensitivity

0.186 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse NF-kappa-B inhibitor beta

Protein Length

359

NCBI Gene Symbol

Nfkbib

Protein Name

Q60778

Gene Name URL

Nfkbib

Nucleotide Accession Number

NM_001306222.1

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