MS4A2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Membrane spanning 4-domains A2
Gene Aliases
APY; ATOPY; Fc epsilon receptor I beta-chain; Fc fragment of IgE, high affinity I, receptor for; beta polypeptide; FCER1B; FCERI; high affinity IgE receptor beta subunit; High affinity immunoglobulin epsilon receptor beta-subunit (FcERI) (IgE Fc receptor, beta-subunit) (Fc epsilon receptor I beta-chain) ; high affinity immunoglobulin epsilon receptor subunit beta; igE Fc receptor subunit beta; IGEL; IGER; IGHER; immunoglobulin E receptor, high affinity, beta polypeptide; membrane-spanning 4-domains subfamily A member 2.
Gene ID
2206
Accession Number
NP_000130.1
Reactivity
Homo sapiens, Human
Target
High affinity immunoglobulin epsilon receptor subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MS4A2 ELISA Kit (Human) (OKEH05309) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MS4A2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MS4A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MS4A2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 94%
Sensitivity
41.3 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human High affinity immunoglobulin epsilon receptor subunit beta
Protein Length
244
NCBI Gene Symbol
MS4A2
Protein Name
Q01362
Gene Name URL
MS4A2
Nucleotide Accession Number
NM_000139.4
Frequently Asked Questions
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