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JMJD6 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Jumonji domain containing 6

Gene Aliases

5730436I23Rik; apoptotic cell clearance receptor PtdSerR; bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; D11Ertd195e; histone arginine demethylase JMJD6; jmjC domain-containing protein 6; jumonji domain-containing protein 6; lysyl-hydroxylase JMJD6; mKIAA0585; peptide-lysine 5-dioxygenase JMJD6; phosphatidylserine receptor; PSR; Pt; Ptd; PtdSerR; Ptdsr.

Gene ID

107817

Accession Number

NP_203971.2

Reactivity

Mouse, Mus musculus

Target

Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Jmjd6 ELISA Kit (Mouse) (OKEH05299) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Jmjd6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Jmjd6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Jmjd6 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 90.3%

Sensitivity

0.399 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6

Protein Length

403

NCBI Gene Symbol

Jmjd6

Protein Name

Q9ERI5

Gene Name URL

Jmjd6

Nucleotide Accession Number

NM_033398.2

Frequently Asked Questions

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