UCP2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Uncoupling protein 2 (mitochondrial, proton carrier)
Gene Aliases
Mitochondrial uncoupling protein 2; Slc25a8; solute carrier family 25 member 8; UCP 2; UCPH; uncoupling protein 2, mitochondrial.
Gene ID
22228
Accession Number
NP_035801.3
Reactivity
Mouse, Mus musculus
Target
Mitochondrial uncoupling protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ucp2 ELISA Kit (Mouse) (OKEH05261) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ucp2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ucp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ucp2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 105.6%
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Mitochondrial uncoupling protein 2
Protein Length
309
NCBI Gene Symbol
Ucp2
Protein Name
P70406
Gene Name URL
Ucp2
CAS Number
7732-18-5
Nucleotide Accession Number
NM_011671.5
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