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KRT5 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Keratin 5

Gene Aliases

3300001P10Rik;58 kDa cytokeratin; AW146334; CK5; cytokeratin 5; Cytokeratin-5; K5; keratin complex 2, basic, gene 5; keratin, type II cytoskeletal 5; Keratin-5; Krt2-; Krt2-5; Tfi; Tfip8; tuftelin interacting protein 8; type-II keratin Kb5.

Gene ID

110308

Accession Number

NP_081287.1

Reactivity

Mouse, Mus musculus

Target

Keratin, type II cytoskeletal 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Krt5 ELISA Kit (Mouse) (OKEH05254) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Krt5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Krt5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Krt5 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 83.8%

Sensitivity

0.192 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Keratin, type II cytoskeletal 5

Protein Length

580

NCBI Gene Symbol

Krt5

Protein Name

Q922U2

Gene Name URL

Krt5

Nucleotide Accession Number

NM_027011.2

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