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ZG16 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Zymogen granule protein 16

Gene Aliases

ZG16p, AI593689, 1810010M01Rik

Gene ID

69036

Accession Number

NP_081194.1

Reactivity

Mouse

Target

May play a role in protein trafficking. May act as a linker molecule between the submembranous matrix on the luminal side of zymogen granule membrane (ZGM) and aggregated secretory proteins during granule formation in the TGN.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Zg16 ELISA Kit (Mouse) (OKEH05217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Zg16 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Zg16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Zg16 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78 - 50ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 101.4%

Sensitivity

0.419 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Zymogen granule membrane protein 16

Protein Length

167

NCBI Gene Symbol

ZG16

Protein Name

Zymogen granule membrane protein 16

Gene Name URL

ZG16

Nucleotide Accession Number

NM_026918.2

Frequently Asked Questions

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