ILF3 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Interleukin enhancer binding factor 3
Gene Aliases
CBTF; Double-stranded RNA-binding protein 76; double-stranded RNA-binding protein, 76 kD; DRBF; DRBP76; dsRNA binding protein NFAR-2/MPP4; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; interleukin enhancer-binding factor 3; M phase phosphoprotein 4, nuclear factor associated with DS RNA; MMP4; M-phase phosphoprotein 4; MPHOSPH4; MPP4; MPP4110; NF110; NF110b; NF90; NF90a; NF90b; NF90c; NF90ctv; NFAR; NFAR-1; NFAR110; NFAR2; NFAR-2; NFAR90; NF-AT-90; nuclear factor associated with dsRNA; nuclear factor of activated T-cells 110 kDa; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP110; TCP80; translational control protein 80.
Gene ID
3609
Accession Number
NP_001131145.1
Reactivity
Homo sapiens, Human
Target
Interleukin enhancer-binding factor 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ILF3 ELISA Kit (Human) (OKEH05183) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ILF3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ILF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ILF3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
7.9 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Interleukin enhancer-binding factor 3
Protein Length
894
NCBI Gene Symbol
ILF3
Protein Name
Q12906
Gene Name URL
ILF3
Nucleotide Accession Number
NM_001137673.1
Frequently Asked Questions
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