UBR1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Ubiquitin protein ligase E3 component n-recognin 1
Gene Aliases
AI504731; E3 alpha; E3 ubiquitin-protein ligase UBR1; N-recognin-1; RING-type E3 ubiquitin transferase UBR1; ubiquitin-protein ligase e3 componen n-recognin; ubiquitin-protein ligase E3-alpha-1; ubiquitin-protein ligase E3-alpha-I.
Gene ID
22222
Accession Number
NP_033487.2
Reactivity
Mouse, Mus musculus
Target
E3 ubiquitin-protein ligase UBR1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ubr1 ELISA Kit (Mouse) (OKEH05169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ubr1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ubr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ubr1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 106.3%
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse E3 ubiquitin-protein ligase UBR1
Protein Length
1757
NCBI Gene Symbol
Ubr1
Protein Name
O70481
Gene Name URL
Ubr1
Nucleotide Accession Number
NM_009461.2
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