GFRA1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Glial cell line derived neurotrophic factor family receptor alpha 1
Gene Aliases
AU042498; GDNF family receptor alpha-1; GDNF receptor alpha 1 (GFRa-1) ; GDNF receptor alpha 1c; GDNF receptor alpha 1d; GDNF receptor alpha 1e; GDNFR; GDNFR-alpha-1; GFR alpha-1; GFRalpha-1; TGF-beta-related neurotrophic factor receptor 1.
Gene ID
14585
Accession Number
NP_001272386.1
Reactivity
Mouse, Mus musculus
Target
GDNF family receptor alpha-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Gfra1 ELISA Kit (Mouse) (OKEH05153) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gfra1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gfra1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gfra1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 110.1%
Sensitivity
39.2 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse GDNF family receptor alpha-1
Protein Length
468
NCBI Gene Symbol
Gfra1
Protein Name
P97785
Gene Name URL
Gfra1
Nucleotide Accession Number
NM_001285457.2
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