PEG10 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Paternally expressed 10
Gene Aliases
AA407948; Ed; Edr; embryonal carcinoma differentiation regulated protein; HB-1; Ma; mammalian retrotransposon-derived protein 2; Mar; Mar2; Mart2; MEF3; MEF3L; MEF3-like protein 1; MyEF-3; MyEF-3 like; myelin expression factor 3; myelin expression factor 3-like protein 1; paternally expressed gene 10 protein; retrotransposon gag domain-containing protein 3; retrotransposon-derived gag-like polyprotein; retrotransposon-derived protein PEG10; Rt; Rtl2; ty3/Gypsy-like protein.
Gene ID
170676
Accession Number
NP_001035701.1
Reactivity
Mouse, Mus musculus
Target
Retrotransposon-derived protein PEG10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Peg10 ELISA Kit (Mouse) (OKEH05137) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Peg10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Peg10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Peg10 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88.2%
Sensitivity
0.092 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Retrotransposon-derived protein PEG10
Protein Length
958
NCBI Gene Symbol
Peg10
Protein Name
Q7TN75
Gene Name URL
Peg10
Nucleotide Accession Number
NM_001040611.1
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