SAE1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
SUMO1 activating enzyme subunit 1
Gene Aliases
2400010M20Rik;2610044L12Rik; A; AL033372; AOS1; AW743391; D7Ertd177; D7Ertd177e; HSPC1; HSPC140; Sua1; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; ubiquitin-like (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A; Uble; Uble1a.
Gene ID
56459
Accession Number
NP_001272820.1
Reactivity
Mouse, Mus musculus
Target
SUMO-activating enzyme subunit 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Sae1 ELISA Kit (Mouse) (OKEH05127) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sae1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sae1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sae1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 94.1%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse SUMO-activating enzyme subunit 1
Protein Length
350
NCBI Gene Symbol
Sae1
Protein Name
Q9R1T2
Gene Name URL
Sae1
Nucleotide Accession Number
NM_001285891.1
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