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FNDC3B ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Fibronectin type III domain containing 3B

Gene Aliases

Factor for adipocyte differentiation 104; FAD104; fibronectin type III domain-containing protein 3B; HCV NS5A-binding protein 37; PRO4979; YVTM2421.

Gene ID

64778

Accession Number

NP_001128567.1

Reactivity

Homo sapiens, Human

Target

Fibronectin type III domain-containing protein 3B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FNDC3B ELISA Kit (Human) (OKEH05108) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FNDC3B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FNDC3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FNDC3B captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 87%

Sensitivity

0.336 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Fibronectin type III domain-containing protein 3B

Protein Length

1204

NCBI Gene Symbol

FNDC3B

Protein Name

Q53EP0

Gene Name URL

FNDC3B

Nucleotide Accession Number

NM_001135095.1

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