Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

WNT7B ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Wingless-type MMTV integration site family, member 7B

Gene Aliases

Protein Wnt-7b; wingless-related MMTV integration site 7B; Wnt-7; Wnt-7b.

Gene ID

22422

Accession Number

NP_001157105.1

Reactivity

Mouse, Mus musculus

Target

Protein Wnt-7b

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Wnt7b ELISA Kit (Mouse) (OKEH05090) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wnt7b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Wnt7b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Wnt7b captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 87.6%

Sensitivity

0.174 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Protein Wnt-7b

Protein Length

349

NCBI Gene Symbol

Wnt7b

Protein Name

P28047

Gene Name URL

Wnt7b

Nucleotide Accession Number

NM_001163633.1

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

FEN1 Recombinant Rabbit mAb
E43S46637 100 µL

FEN1 Recombinant Rabbit mAb

Sign In for Pricing
View Details
Lentiviral mouse Dhcr7 shRNA (UAS) - Lentiviral mouse Dhcr7 shRNA (UAS, GFP) plasmid
GTR15186730 1 Vial

Lentiviral mouse Dhcr7 shRNA (UAS) - Lentiviral mouse Dhcr7 shRNA (UAS, GFP) plasmid

Sign In for Pricing
View Details
BC050099 (BC050099) Mouse Tagged ORF Clone
MR207606 10 µg

BC050099 (BC050099) Mouse Tagged ORF Clone

Sign In for Pricing
View Details
ALKBH2 Rabbit mAb
MBS4761339-01 0.1 mL

ALKBH2 Rabbit mAb

Sign In for Pricing
View Details
ALKBH2 Rabbit mAb
MBS4761339-02 5x 0.1 mL

ALKBH2 Rabbit mAb

Sign In for Pricing
View Details
TMTD (Tetramethylthiuram disulfide)
orb2893742-01 500 mg

TMTD (Tetramethylthiuram disulfide)

Sign In for Pricing
View Details