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LILRB4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Leukocyte immunoglobulin-like receptor, subfamily B, member 4A

Gene Aliases

CD85K; glycoprotein 49 B; gp49; Gp49b; HM18; IL; ILT3; leukocyte immunoglobulin-like receptor subfamily B member 4; leukocyte immunoglobulin-like receptor, subfamily B, member 4; Li; Lilrb4; LIR-5; mast cell surface glycoprotein Gp49B.

Gene ID

14728

Accession Number

NP_038560.1

Reactivity

Mouse, Mus musculus

Target

Leukocyte immunoglobulin-like receptor subfamily B member 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Lilrb4 ELISA Kit (Mouse) (OKEH05081) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lilrb4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lilrb4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lilrb4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 113%

Sensitivity

0.177 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Leukocyte immunoglobulin-like receptor subfamily B member 4

Protein Length

335

NCBI Gene Symbol

Lilrb4a

Protein Name

Q64281

Gene Name URL

Lilrb4a

Nucleotide Accession Number

NM_013532.3

Frequently Asked Questions

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