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PLPPR4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Phospholipid phosphatase related 4

Gene Aliases

L, PRG, Lppr4, PRG-1, mKIAA0455, A330086D10, D3Bwg0562e

Gene ID

229791

Accession Number

NP_808332.3

Reactivity

Mouse

Target

Hydrolyzes lysophosphatidic acid (LPA) . Facilitates axonal outgrowth during development and regenerative sprouting. In the outgrowing axons acts as an ecto-enzyme and attenuates phospholipid-induced axon collapse in neurons and facilitates outgrowth in the hippocampus.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Plppr4 ELISA Kit (Mouse) (OKEH05079) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Plppr4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Plppr4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plppr4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78 - 50ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 83.2%

Sensitivity

0.39 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Lipid phosphate phosphatase-related protein type 4

Protein Length

766

NCBI Gene Symbol

PLPPR4

Protein Name

Phospholipid phosphatase-related protein type 4

Gene Name URL

PLPPR4

Nucleotide Accession Number

NM_177664.5

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