PLPPR4 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Phospholipid phosphatase related 4
Gene Aliases
L, PRG, Lppr4, PRG-1, mKIAA0455, A330086D10, D3Bwg0562e
Gene ID
229791
Accession Number
NP_808332.3
Reactivity
Mouse
Target
Hydrolyzes lysophosphatidic acid (LPA) . Facilitates axonal outgrowth during development and regenerative sprouting. In the outgrowing axons acts as an ecto-enzyme and attenuates phospholipid-induced axon collapse in neurons and facilitates outgrowth in the hippocampus.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Plppr4 ELISA Kit (Mouse) (OKEH05079) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Plppr4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Plppr4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plppr4 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78 - 50ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 83.2%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Lipid phosphate phosphatase-related protein type 4
Protein Length
766
NCBI Gene Symbol
PLPPR4
Protein Name
Phospholipid phosphatase-related protein type 4
Gene Name URL
PLPPR4
Nucleotide Accession Number
NM_177664.5
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