PIP4K2C ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Gene Aliases
Pip5, Pip5k2c
Gene ID
117150
Accession Number
NP_473438.1
Reactivity
Mouse
Target
May play an important role in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Pip4k2c ELISA Kit (Mouse) (OKEH05057) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pip4k2c has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pip4k2c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pip4k2c captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156 - 10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 99.2%
Sensitivity
0.084 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Protein Length
421
NCBI Gene Symbol
PIP4K2C
Protein Name
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Gene Name URL
PIP4K2C
Nucleotide Accession Number
NM_054097.3
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