EFHD2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
EF-hand domain-containing protein D2
Gene Aliases
D4Wsu27, AA408606, D4Wsu27e, 2600015J22Rik
Gene ID
27984
Accession Number
NP_080270.2
Reactivity
Mouse
Target
May regulate B-cell receptor (BCR) -induced immature and primary B-cell apoptosis. Plays a role as negative regulator of the canonical NF-kappa-B-activating branch. Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Efhd2 ELISA Kit (Mouse) (OKEH05046) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Efhd2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Efhd2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Efhd2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78 - 50ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 84.5%
Sensitivity
0.399 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse EF-hand domain-containing protein D2
Protein Length
240
NCBI Gene Symbol
EFHD2
Protein Name
EF-hand domain-containing protein D2
Gene Name URL
EFHD2
Nucleotide Accession Number
NM_025994.3
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