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ZAP70 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Zeta-chain (TCR) associated protein kinase

Gene Aliases

70 kDa zeta-chain associated protein; mrtle; mur; S; Srk; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; ZAP-; ZAP-70.

Gene ID

22637

Accession Number

NP_001276541.1

Reactivity

Mouse, Mus musculus

Target

Tyrosine-protein kinase ZAP-70

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Zap70 ELISA Kit (Mouse) (OKEH05039) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Zap70 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Zap70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Zap70 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 84.2%

Sensitivity

0.315 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Tyrosine-protein kinase ZAP-70

Protein Length

618

NCBI Gene Symbol

Zap70

Protein Name

P43404

Gene Name URL

Zap70

Nucleotide Accession Number

NM_001289612.1

Frequently Asked Questions

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