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C8B ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Complement component 8, beta polypeptide

Gene Aliases

4930439B20Rik; AI595927; Complement component 8 subunit beta; complement component 8, beta subunit; complement component C8 beta chain.

Gene ID

110382

Accession Number

NP_001303600.1

Reactivity

Mouse, Mus musculus

Target

Complement component C8 beta chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology C8b ELISA Kit (Mouse) (OKEH04997) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C8b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C8b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C8b captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 80%

Sensitivity

41.2 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Complement component C8 beta chain

Protein Length

589

NCBI Gene Symbol

C8b

Protein Name

Q8BH35

Gene Name URL

C8b

Nucleotide Accession Number

NM_001316671.1

Frequently Asked Questions

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