HMGN1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
High mobility group nucleosomal binding domain 1
Gene Aliases
High mobility group nucleosome-binding domain-containing protein 1; high mobility group protein 14; Hmg14; HMG-14; non-histone chromosomal protein HMG-14.
Gene ID
15312
Accession Number
NP_032277.3
Reactivity
Mouse, Mus musculus
Target
Non-histone chromosomal protein HMG-14
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hmgn1 ELISA Kit (Mouse) (OKEH04983) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hmgn1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hmgn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hmgn1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 95.8%
Sensitivity
0.179 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Non-histone chromosomal protein HMG-14
Protein Length
96
NCBI Gene Symbol
Hmgn1
Protein Name
P18608
Gene Name URL
Hmgn1
Nucleotide Accession Number
NM_008251.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
