GSTA2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Glutathione S-transferase, alpha 2 (Yc2)
Gene Aliases
Glutathione S-transferase A2; glutathione S-transferase GT41A; GST class-alpha member 2; Gst2-; Gst2-2; Gstc; Gstc-; Gstc2; Gstc-2.
Gene ID
14858
Accession Number
NP_032208.2
Reactivity
Mouse, Mus musculus
Target
Glutathione S-transferase A2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Gsta2 ELISA Kit (Mouse) (OKEH04982) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gsta2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gsta2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gsta2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 96.2%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Glutathione S-transferase A2
Protein Length
222
NCBI Gene Symbol
Gsta2
Protein Name
P10648
Gene Name URL
Gsta2
CAS Number
7732-18-5
Nucleotide Accession Number
NM_008182.3
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