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GSTA2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Glutathione S-transferase, alpha 2 (Yc2)

Gene Aliases

Glutathione S-transferase A2; glutathione S-transferase GT41A; GST class-alpha member 2; Gst2-; Gst2-2; Gstc; Gstc-; Gstc2; Gstc-2.

Gene ID

14858

Accession Number

NP_032208.2

Reactivity

Mouse, Mus musculus

Target

Glutathione S-transferase A2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Gsta2 ELISA Kit (Mouse) (OKEH04982) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gsta2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gsta2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gsta2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 96.2%

Sensitivity

0.087 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Glutathione S-transferase A2

Protein Length

222

NCBI Gene Symbol

Gsta2

Protein Name

P10648

Gene Name URL

Gsta2

Nucleotide Accession Number

NM_008182.3

Frequently Asked Questions

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