PPP1CA ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Protein phosphatase 1 catalytic subunit alpha
Gene Aliases
Dis; dism2; Pp; PP-1A; Ppp1c; serine/threonine-protein phosphatase PP1-alpha catalytic subunit.
Gene ID
19045
Accession Number
NP_114074.1
Reactivity
Mouse, Mus musculus
Target
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ppp1ca ELISA Kit (Mouse) (OKEH04977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppp1ca has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppp1ca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppp1ca captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.7%
Sensitivity
0.17 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Protein Length
330
NCBI Gene Symbol
Ppp1ca
Protein Name
P62137
Gene Name URL
Ppp1ca
Nucleotide Accession Number
NM_031868.2
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