What is the catalog number for PPP1CA ELISA Kit (Mouse)?

The catalog number for PPP1CA ELISA Kit (Mouse) is OKEH04977-96W.

What sizes are available for PPP1CA ELISA Kit (Mouse)?

PPP1CA ELISA Kit (Mouse) is available in the following size(s): 96 Tests.

Is PPP1CA ELISA Kit (Mouse) in stock?

PPP1CA ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

PPP1CA ELISA Kit (Mouse)

Selected Variant

OKEH04977-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Protein phosphatase 1 catalytic subunit alpha

Gene Aliases

Dis; dism2; Pp; PP-1A; Ppp1c; serine/threonine-protein phosphatase PP1-alpha catalytic subunit.

Gene ID

19045

Accession Number

NP_114074.1

Reactivity

Mouse, Mus musculus

Target

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ppp1ca ELISA Kit (Mouse) (OKEH04977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppp1ca has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppp1ca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppp1ca captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 89.7%

Sensitivity

0.17 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Protein Length

330

NCBI Gene Symbol

Ppp1ca

Protein Name

P62137

Gene Name URL

Ppp1ca

Nucleotide Accession Number

NM_031868.2

Price

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