What is the catalog number for DGKA ELISA Kit (Mouse)?

The catalog number for DGKA ELISA Kit (Mouse) is OKEH04965-96W.

What sizes are available for DGKA ELISA Kit (Mouse)?

DGKA ELISA Kit (Mouse) is available in the following size(s): 96 Tests.

Is DGKA ELISA Kit (Mouse) in stock?

DGKA ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

DGKA ELISA Kit (Mouse)

Selected Variant

OKEH04965-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Diacylglycerol kinase, alpha

Gene Aliases

80 kDa diacylglycerol kinase;80kDa; AW146112; DAG kinase alpha; Dagk; Dagk1; DGK-alpha; diacylglycerol kinase alpha; diacylglycerol kinase alpha Flt2-Pr2; diglyceride kinase alpha; truncated diacylglycerol kinase alpha Flt1-Pr1; truncated diacylglycerol kinase alpha Flt1-Pr2.

Gene ID

13139

Accession Number

NP_058091.2

Reactivity

Mouse, Mus musculus

Target

Diacylglycerol kinase alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Dgka ELISA Kit (Mouse) (OKEH04965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dgka has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dgka is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dgka captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 81.3%

Sensitivity

0.316 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Diacylglycerol kinase alpha

Protein Length

730

NCBI Gene Symbol

Dgka

Protein Name

O88673

Gene Name URL

Dgka

Nucleotide Accession Number

NM_016811.2

Price

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