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SCAMP1 ELISA Kit (Mouse)

Selected Variant

OKEH04952-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Secretory carrier membrane protein 1

Gene Aliases

4930505M11Rik; AI415563; AW122395; secretory carrier-associated membrane protein 1.

Gene ID

107767

Accession Number

NP_083429.1

Reactivity

Mouse, Mus musculus

Target

Secretory carrier-associated membrane protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Scamp1 ELISA Kit (Mouse) (OKEH04952) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Scamp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Scamp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Scamp1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 95.1%

Sensitivity

40 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Secretory carrier-associated membrane protein 1

Protein Length

338

NCBI Gene Symbol

Scamp1

Protein Name

Q8K021

Gene Name URL

Scamp1

Nucleotide Accession Number

NM_029153.1

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