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H3F3C ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

H3.3 histone A, H3.3 histone B

Gene Aliases

9430068D06Rik; EyeLinc14; H3 histone, family 3A; H3 histone, family 3B; H3.3; H3.3A; H3.3B; H3-3; H3-3a; H3-3b; H3L-like histone; histone H3.3.

Gene ID

15078, 15081

Accession Number

XP_894986.1

Reactivity

Mouse, Mus musculus

Target

Histone H3.3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology H3f3c ELISA Kit (Mouse) (OKEH04942) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H3f3c has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H3f3c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H3f3c captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 104.6%

Sensitivity

0.182 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Histone H3.3

Protein Length

136

NCBI Gene Symbol

H3f3a, H3f3b

Protein Name

P84244

Gene Name URL

H3f3a, H3f3b

Nucleotide Accession Number

XM_889893.7

Frequently Asked Questions

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