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MAU ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Annexin A6

Gene Aliases

67 kDa calelectrin; annexin A6; Annexin VI; annexin VI (p68) ; annexin-6; ANX6; calcium-binding protein p68; calelectrin; calphobindin II; Calphobindin-II; CBP68; chromobindin-20; CPB-II; lipocortin VI; p68; p70; Protein III; testis secretory sperm-binding protein Li 198a.

Gene ID

309

Accession Number

NP_001146.2

Reactivity

Homo sapiens, Human

Target

Annexin A6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MAU ELISA Kit (Human) (OKEH04873) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAU has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAU is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAU captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/ml

Sensitivity

0.098 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

76 kDa

Specificity

Natural and recombinant Human Annexin A6

Protein Length

673

NCBI Gene Symbol

ANXA6

Protein Name

P08133

Gene Name URL

ANXA6

Nucleotide Accession Number

NM_001155.4

Frequently Asked Questions

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